ht-22 cells  (Procell Inc)

Journal: Cell Death Discovery

Article Title: The role of hydroxyindoles in protecting neuronal cultures from ferroptosis

doi: 10.1038/s41420-025-02608-4

Figure Lengend Snippet: A cell-free ABTS assay was performed to evaluate the RTA properties, measured as the percentage of radical scavenging activity ( A ). Cell viability, ATP levels, and GSH concentration were assessed after co-treatment with various compounds for 24 h, supplemented with Lip-1 (1 µM) to prevent ferroptosis in HT-22 cells ( B , C ). The compounds used included 25 µM of 3-hydroxyindole (3-HI), 6-hydroxyindole (6-HI), and 7-hydroxyindole (7-HI). These treatments were co-treated with the inducers erastin (1 µM) and rotenone (20 µM) ( B , C ). The cell viability results indicated no ferroptotic cytotoxicity ( B , C ). Cell viability was evaluated using the calcein AM assay, ATP levels were measured with the ATP-Glo™ bioluminometric assay, and GSH levels were assessed using an mBCI-based assay ( B , C ). Data points represent the mean percentage survival compared to untreated cells ± SEM, with n = 12 from 3 independent experiments.

Article Snippet: The HT-22 mouse hippocampal cell line was subcloned from the HT-4 cell line, which was generously provided by Dr. Val J. Watts, Purdue University, USA.

Techniques: ABTS Assay, Activity Assay, Concentration Assay, Calcein AM Assay